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验证数据展示

  • WB analysis using 24337-1-AP

    WB analysis using 24337-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24337-1-AP (BBOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 24337-1-AP

    IHC staining of mouse testis using 24337-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 24337-1-AP

    IHC staining of mouse testis using 24337-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BBOF1 Polyclonal antibody

BBOF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24337-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C14orf45, Coiled-coil domain-containing protein 176, CCDC176, Basal body-orientation factor 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, TT cells, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24337-1-AP targets BBOF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BBOF1 fusion protein Ag19302 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 45
别名 C14orf45, Coiled-coil domain-containing protein 176, CCDC176, Basal body-orientation factor 1
计算分子量 529 aa, 62 kDa
观测分子量62 kDa
GenBank蛋白编号BC104981
基因名称 BBOF1
Gene ID (NCBI) 80127
RRIDAB_3669433
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8ND07
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Basal body orientation factor 1 (BBOF1), also known as CCDC176, contains two coiled-coil domains and is evolutionarily conserved in vertebrates. Recently, Xenopus bbof1 has been characterized as a cilia-associated factor that is induced by the transcription factor foxj1 to mediate the alignment and orientation of cilia in epidermal multi-ciliated cells. BBOF1 is required for sperm flagellar axoneme maintenance and male fertility in mice. BBOF1 expression is elevated during meiosis, and it is accumulated in round spermatids. BBOF1 deletion has compromised effects on growth, survival, and spermatogenesis.

实验方案

Product Specific Protocols
WB protocol for BBOF1 antibody 24337-1-APDownload protocol
IHC protocol for BBOF1 antibody 24337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols