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验证数据展示

  • IF Staining of hTERT-RPE1 cells using 21118-1-AP

    IF Staining of hTERT-RPE1 cells using 21118-1-AP

    IF result (base of ciliary stain) of anti-BBS1 (21118-1-AP, 1:50) with with serum-starved hTERT-RPE1 cells by Dr. Moshe Kim.

  • WB analysis of mouse kidney using 21118-1-AP

    WB analysis of mouse kidney using 21118-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21118-1-AP

    IP experiment of mouse brain using 21118-1-AP

    IP result of anti-BBS1 (IP:21118-1-AP, 4ug; Detection:21118-1-AP 1:600) with mouse brain tissue lysate 4000ug.

BBS1 Polyclonal antibody

BBS1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 21118-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA and More (1)

别名

Bardet Biedl syndrome 1, BBS1, BBS2 like protein 2, BBS2L2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse brain tissue, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21118-1-AP targets BBS1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 BBS1 fusion protein Ag15460 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Bardet-Biedl syndrome 1
别名 Bardet Biedl syndrome 1, BBS1, BBS2 like protein 2, BBS2L2
计算分子量 593 aa, 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC047642
基因名称 BBS1
Gene ID (NCBI) 582
RRIDAB_2878814
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFJ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BBS1 antibody 21118-1-APDownload protocol
IP protocol for BBS1 antibody 21118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan
View Article
humanIF

J Biol Chem

Non-redundant roles of DIAPHs in primary ciliogenesis.

Authors - Oliva Palander
View Article

PLoS Biol

Melanocortin 1 receptor mediates melanin production by interacting with the BBSome in primary cilia

Authors - Xiaoyu Tian
View Article