验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, A549 cells, MCF-7 cells |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12421-2-AP targets BBS10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | BBS10 fusion protein Ag3073 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Bardet-Biedl syndrome 10 |
| 别名 | C12orf58, Bardet-Biedl syndrome 10 protein, Bardet Biedl syndrome 10 |
| 计算分子量 | 81 kDa |
| 观测分子量 | 81 kDa |
| GenBank蛋白编号 | BC013795 |
| 基因名称 | BBS10 |
| Gene ID (NCBI) | 79738 |
| RRID | AB_2064962 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TAM1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BBS10 antibody 12421-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Transient ciliogenesis involving Bardet-Biedl syndrome proteins is a fundamental characteristic of adipogenic differentiation.
| ||
Proc Natl Acad Sci U S A BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. | ||
Cilia Comparing the Bbs10 complete knockout phenotype with a specific renal epithelial knockout one highlights the link between renal defects and systemic inactivation in mice.
|
