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验证数据展示

  • WB analysis using 66518-1-Ig

    WB analysis using 66518-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66518-1-Ig

    IHC staining of human liver cancer using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66518-1-Ig

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66518-1-Ig

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MKS1 Monoclonal antibody

MKS1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66518-1-Ig

产品说明书

CloneNo. 6A8B11

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, rat, mouse

应用

WB, IHC, ELISA

别名

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCCIT cells, pig brain tissue, HEK-293 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66518-1-Ig targets MKS1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, pig, rat, mouse
免疫原 MKS1 fusion protein Ag9504 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Meckel syndrome, type 1
别名 BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1
计算分子量 559 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC010061
基因名称 MKS1
Gene ID (NCBI) 54903
RRIDAB_2881881
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NXB0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MKS1 antibody 66518-1-IgDownload protocol
IHC protocol for MKS1 antibody 66518-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols