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MKS1 Monoclonal antibody

MKS1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6A8B11

Cat no : 66518-1-Ig

Print datasheet

Synonyms

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1

验证数据展示

  • WB analysis using 66518-1-Ig

    WB analysis using 66518-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66518-1-Ig

    IHC staining of human liver cancer using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66518-1-Ig

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66518-1-Ig

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNCCIT cells, pig brain tissue, HEK-293 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66518-1-Ig targets MKS1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, pig, rat, mouse
Immunogen MKS1 fusion protein Ag9504 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Meckel syndrome, type 1
Synonyms BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1
Calculated Molecular Weight 559 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC010061
Gene Symbol MKS1
Gene ID (NCBI) 54903
RRIDAB_2881881
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NXB0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MKS1 antibody 66518-1-IgDownload protocol
IHC protocol for MKS1 antibody 66518-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols