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BBS6 Polyclonal antibody

BBS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13078-1-AP

Print datasheet

Synonyms

MKKS, MKS, McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin, KMS, HMCS

验证数据展示

  • IHC staining of mouse testis using 13078-1-AP

    IHC staining of mouse testis using 13078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MKKS antibody included
  • IHC staining of mouse testis using 13078-1-AP

    IHC staining of mouse testis using 13078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MKKS antibody included
  • IF Staining of hTERT-RPE1 cells using 13078-1-AP

    IF Staining of hTERT-RPE1 cells using 13078-1-AP

    IF result (cytoplasm and the base of cilia stain) of anti-BBS6 (13078-1-AP; 1:50) with hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

  • WB analysis of mouse testis using 13078-1-AP

    WB analysis of mouse testis using 13078-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13078-1-AP (BBS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse testis tissue, mouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy MKKS Ready-To-Use IHC Kit. MKKS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13078-1-AP targets BBS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen BBS6 fusion protein Ag3785 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name McKusick-Kaufman syndrome
Synonyms MKKS, MKS, McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin, KMS, HMCS
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC028973
Gene Symbol MKKS
Gene ID (NCBI) 8195
RRIDAB_10603370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPJ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MKKS also known as BBS6 is a probable chaperone given to the amino acid similarity to the chaperonin family of proteins and may play a role in protein processing in limb, cardiac and reproductive system development. The mutations in BBS6 have been linked to Bardet-Biedl syndrome (BBS) which is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. It may also get involved in cellular organization processes, in particular relating to ciliary/flagellar and centrosomal activities.

实验方案

Product Specific Protocols
WB protocol for BBS6 antibody 13078-1-APDownload protocol
IHC protocol for BBS6 antibody 13078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Neurobiol

Inpp5e Regulated the Cilium-Related Genes Contributing to the Neural Tube Defects Under 5-Fluorouracil Exposure

Authors - Xiuwei Wang
View Article