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BAP29 Polyclonal antibody

BAP29 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 15796-1-AP

Print datasheet

Synonyms

BCR-associated protein 29, B-cell receptor-associated protein 29, BCAP29

验证数据展示

  • WB analysis using 15796-1-AP

    WB analysis using 15796-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 15796-1-AP

    IHC staining of human lymphoma using 15796-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human lymphoma using 15796-1-AP

    IHC staining of human lymphoma using 15796-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 10x lens).

  • IF Staining of HeLa using 15796-1-AP

    IF Staining of HeLa using 15796-1-AP

    Immunofluorescent analysis of Hela cells, using BCAP29 antibody 15796-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • FC experiment of HEK-293 using 15796-1-AP

    FC experiment of HEK-293 using 15796-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug BAP29 Polyclonal antibody (15796-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inJurkat cells, COLO 320 cells, Raji cells, U-87 MG cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15796-1-AP targets BAP29 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen BAP29 fusion protein Ag8505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell receptor-associated protein 29
Synonyms BCR-associated protein 29, B-cell receptor-associated protein 29, BCAP29
Calculated Molecular Weight 241 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC008478
Gene Symbol BAP29
Gene ID (NCBI) 55973
RRIDAB_2065118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BAP29 antibody 15796-1-APDownload protocol
IHC protocol for BAP29 antibody 15796-1-APDownload protocol
IF protocol for BAP29 antibody 15796-1-APDownload protocol
FC protocol for BAP29 antibody 15796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols