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BCAR3 Polyclonal antibody

BCAR3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13628-1-AP

Print datasheet

Synonyms

BCAR3, KIAA0554, Novel SH2 containing protein 2, NSP2, SH2D3B

验证数据展示

  • WB analysis of K-562 using 13628-1-AP

    WB analysis of K-562 using 13628-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13628-1-AP (BCAR3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat adrenal gland using 13628-1-AP

    IHC staining of rat adrenal gland using 13628-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 13628-1-AP (BCAR3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inrat adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13628-1-AP targets BCAR3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen BCAR3 fusion protein Ag4546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name breast cancer anti-estrogen resistance 3
Synonyms BCAR3, KIAA0554, Novel SH2 containing protein 2, NSP2, SH2D3B
Calculated Molecular Weight 825 aa, 93 kDa
Observed Molecular Weight 92-105 kDa
GenBank Accession NumberBC039895
Gene Symbol BCAR3
Gene ID (NCBI) 8412
RRIDAB_2877962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75815
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BCAR3 antibody 13628-1-APDownload protocol
IHC protocol for BCAR3 antibody 13628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Int J Nanomedicine

Localized Administration of Bcar3 siRNA via Nano-Self-Assembly to Treat Idiopathic Pulmonary Fibrosis by Disrupting Macrophage-Fibroblast Crosstalk

Authors - Chenxi Zeng
View Article