验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, mouse liver tissue, hESC cells, human brain tissue, K-562 cells, COS-7 cells, SH-SY5Y cells, PC-12 cells, C6 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10414-1-AP targets BCAS2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, RIP |
| 经测试反应性 | human, mouse, rat, monkey |
| 文献引用反应性 | human, mouse |
| 免疫原 | BCAS2 fusion protein Ag0669 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | breast carcinoma amplified sequence 2 |
| 别名 | Spliceosome-associated protein SPF 27, Pre-mRNA-splicing factor SPF27, Pre mRNA splicing factor SPF27, DAM1, Breast carcinoma-amplified sequence 2 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26-32 kDa |
| GenBank蛋白编号 | BC005285 |
| 基因名称 | BCAS2 |
| Gene ID (NCBI) | 10286 |
| RRID | AB_2063400 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75934 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCAS2 associates with the spliceosome and implicates in mRNA splicing
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BCAS2 antibody 10414-1-AP | Download protocol |
| IHC protocol for BCAS2 antibody 10414-1-AP | Download protocol |
| IF protocol for BCAS2 antibody 10414-1-AP | Download protocol |
| IP protocol for BCAS2 antibody 10414-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. | ||
Int J Biochem Cell Biol BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination. | ||
Stem Cell Res Ther Breast carcinoma-amplified sequence 2 regulates adult neurogenesis via β-catenin.
| ||
Br J Cancer BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability.
| ||
FASEB J BCAS2 is involved in alternative splicing and mouse oocyte development.
| ||
Endocrinology BCAS2 participates in insulin synthesis and secretion via mRNA alternative splicing in mice |
