验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, K-562 cells, Jurkat cells, A431 cells, HEK-293 cells, A549 cells, LNCaP cells, pig brain tissue, rat brain tissue, mouse rat tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67085-1-Ig targets BCAS3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Mouse, Rat, Pig |
| 免疫原 | BCAS3 fusion protein Ag28545 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | breast carcinoma amplified sequence 3 |
| 别名 | BCAS3, DKFZp686O1527, FLJ20128, GAOB1, MAAB, Protein Maab1 |
| 计算分子量 | 99 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC001250 |
| 基因名称 | BCAS3 |
| Gene ID (NCBI) | 54828 |
| RRID | AB_2882393 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H6U6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rudhira/BCAS3 (Breast Cancer Amplified Sequence 3) is a conserved protein expressed in the embryonic vasculature and malignant tumors. Rudhira is a predicted WD40 domain protein. During mouse development Rudhira is expressed in erythropoietic and angiogenic precursors but not in their differentiated progeny. This transient expression of Rudhira is also seen in normal adult angiogenesis. BCAS3, the human ortholog of Rudhira is 98% identical and the gene maps to a breakpoint of hematological neoplasms on chromosome 17q23.1. BCAS3 is also expressed in vascular development in embryoid bodies in vitro and was originally identified as a gene over-expressed in breast cancers. It is reported to be a target of metastasis associated protein-1 (MTA-1). Further BCAS3 expression is also upregulated in grade III and IV glioblastomas where it is misexpressed in the tumor cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BCAS3 antibody 67085-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
