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BCAT1 Polyclonal antibody

BCAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28622-1-AP

Print datasheet

Synonyms

BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39

验证数据展示

  • WB analysis using 28622-1-AP

    WB analysis using 28622-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28622-1-AP (BCAT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 28622-1-AP

    IHC staining of human liver cancer using 28622-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inC6 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28622-1-AP targets BCAT1 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, rat
Cited Reactivityhuman, mouse
Immunogen BCAT1 fusion protein Ag28998 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name branched chain aminotransferase 1, cytosolic
Synonyms BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39
Calculated Molecular Weight 320 aa, 36 kDa
Observed Molecular Weight 43-48 kDa
GenBank Accession NumberBC033864
Gene Symbol BCAT1
Gene ID (NCBI) 586
RRIDAB_2881183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54687
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

实验方案

Product Specific Protocols
WB protocol for BCAT1 antibody 28622-1-APDownload protocol
IHC protocol for BCAT1 antibody 28622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Oncol Rep

BCAT1 overexpression regulates proliferation and c‑Myc/GLUT1 signaling in head and neck squamous cell carcinoma.

Authors - Hongming Wang
View Article
mouseWB,IHC

Biochim Biophys Acta Mol Basis Dis

Targeted inhibition of branched-chain amino acid metabolism drives apoptosis of glioblastoma by facilitating ubiquitin degradation of Mfn2 and oxidative stress

Authors - Zhuo Lu
View Article