验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16043-1-AP targets BCCIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | BCCIP fusion protein Ag8934 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BRCA2 and CDKN1A interacting protein |
| 别名 | TOK1, TOK 1, Protein TOK-1, Protein TOK 1, P21- and CDK-associated protein 1 |
| 计算分子量 | 314 aa, 36 kDa |
| 观测分子量 | 50-58 kDa |
| GenBank蛋白编号 | BC009771 |
| 基因名称 | BCCIP |
| Gene ID (NCBI) | 56647 |
| RRID | AB_2063712 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P287 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BCCIP antibody 16043-1-AP | Download protocol |
| IF protocol for BCCIP antibody 16043-1-AP | Download protocol |
| IP protocol for BCCIP antibody 16043-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci The Males Absent on the First (MOF) Mediated Acetylation Alters the Protein Stability and Transcriptional Activity of YY1 in HCT116 Cells | ||
Int J Mol Sci Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells | ||
FEBS J BCCIP binds to and activates its promoter in a YY1-dependent fashion in HCT116 cells.
| ||
Protein Cell Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein (BCCIP) in cells. | ||
Biochem Biophys Res Commun BCCIPβ facilitates p53 ubiquitination via binding with E6 protein in high-risk HPV positive head and neck squamous cell carcinoma. | ||
Int J Oncol Differential BCCIP gene expression in primary human ovarian cancer, renal cell carcinoma and colorectal cancer tissues. |
