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验证数据展示

  • WB analysis using 30028-1-AP

    WB analysis using 30028-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30028-1-AP (BCKDHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 30028-1-AP

    IHC staining of human liver using 30028-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30028-1-AP (BCKDHA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCKDHA antibody included
  • WB analysis of HepG2 using 30028-1-AP

    WB analysis of HepG2 using 30028-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 30028-1-AP (BCKDHA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 30028-1-AP

    IHC staining of human liver using 30028-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30028-1-AP (BCKDHA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCKDHA antibody included
  • Featured Product
  • KD/KO Validated

BCKDHA Polyclonal antibody

BCKDHA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30028-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

BCKDHA, FLJ45695, MSU, MSUD1, OVD1A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BCKDHA Ready-To-Use IHC Kit. BCKDHA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30028-1-AP targets BCKDHA in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivitymouse, rat
Immunogen BCKDHA fusion protein Ag32613 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name branched chain keto acid dehydrogenase E1, alpha polypeptide
Synonyms BCKDHA, FLJ45695, MSU, MSUD1, OVD1A
Calculated Molecular Weight 50 kDa
Observed Molecular Weight42-50 kDa
GenBank Accession NumberBC008933
Gene Symbol BCKDHA
Gene ID (NCBI) 593
RRIDAB_2935504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12694
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), the gene encoding the regulated subunit of BCKDC was only one of two primary susceptibility genes identified that affected the risk of both type 2 diabetes mellitus (T2DM) and obesity (PMID: 25287287). BIX01294 transcriptionally downregulated the transcription of BCKDHA, which is essential for fueling the tricarboxylic acid (TCA) cycle. Studies have shown that KDM3A, a Jumonji histone demethylase, epigenetically regulates BCKDHA expression by binding to the BCKDHA gene promoter (PMID: 34876693). Moreover, at least four genes including BCKDHA, branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), dihydrolipoamide dehydrogenase (DLD), and dihydrolipoamide branched chain transacylase E2 (DBT) have been reported to be the causative gene for Maple syrup urine disease (MSUD) (PMID: 34187135).

实验方案

Product Specific Protocols
WB protocol for BCKDHA antibody 30028-1-APDownload protocol
IHC protocol for BCKDHA antibody 30028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Clin Nutr

The effects of vitamin B6 on the nutritional support of BCAAs-enriched amino acids formula in rats with partial gastrectomy

Authors - Ying Wang
View Article
mouseWB

J Thromb Haemost

Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling

Authors - Haojie Jiang
  • KO Validated
View Article