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验证数据展示

  • Cytometric bead array standard curve of MP50570-1

    Cytometric bead array standard curve of MP50570-1

    Cytometric bead array standard curve of MP50570-1, BCKDHB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68596-2-PBS. Detection antibody: 68596-1-PBS. Standard:Ag33802. Range: 0.391-100 ng/mL.

  • Cytometric bead array standard curve of MP50570-3

    Cytometric bead array standard curve of MP50570-3

    Cytometric bead array standard curve of MP50570-3, BCKDHB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68596-4-PBS. Detection antibody: 68596-1-PBS. Standard:Ag33802. Range: 1.563-100 ng/mL.

BCKDHB Monoclonal antibody, PBS Only (Detector)

BCKDHB Monoclonal Antibody for Cytometric bead array, Indirect ELISA
Cat No. 68596-1-PBS

产品说明书

CloneNo. 1D12C7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

Cytometric bead array, Indirect ELISA

别名

E1B, Branched-chain alpha-keto acid dehydrogenase E1 component beta chain, BCKDH E1-beta, BCKDE1B, 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68596-1-PBS targets BCKDHB as part of a matched antibody pair:

MP50570-1: 68596-2-PBS capture and 68596-1-PBS detection (validated in Cytometric bead array)

MP50570-3: 68596-4-PBS capture and 68596-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BCKDHB fusion protein Ag33802 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 branched chain keto acid dehydrogenase E1, beta polypeptide
别名 E1B, Branched-chain alpha-keto acid dehydrogenase E1 component beta chain, BCKDH E1-beta, BCKDE1B, 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
计算分子量 392 aa, 43 kDa
观测分子量35-37 kDa
GenBank蛋白编号BC040139
基因名称 BCKDHB
Gene ID (NCBI) 594
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP21953
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Branched-chain alpha-keto acid dehydrogenase E1 component beta chain (BCKDHB; BCKDE1B; BCKDH E1-beta) is a subunit of the BCKDH complex, which is a mitochondrial enzyme in the degradation pathway for branched-chain amino acids (BCAA). Together with BCKDHA, BCKDHB forms the E1 subunit of this complex, whereas DBT and DLD are the E2 and E3 subunits, respectively. A deficiency of the BCKDH complex in humans causes maple syrup urine disease (MSUD), a severe neurometabolic disorder diagnosed by the detection alloisoleucine in plasma (MIM 248600). (PMID: 30709776)