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验证数据展示

  • WB analysis using 15718-1-AP

    WB analysis using 15718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 15718-1-AP

    IHC staining of mouse pancreas using 15718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCKDK antibody included
  • IHC staining of mouse pancreas using 15718-1-AP

    IHC staining of mouse pancreas using 15718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCKDK antibody included

BCKDK Polyclonal antibody

BCKDK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15718-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

BCKDK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BCKDK Ready-To-Use IHC Kit. BCKDK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15718-1-AP targets BCKDK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 BCKDK fusion protein Ag8395 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 branched chain ketoacid dehydrogenase kinase
别名 BCKDK
计算分子量 412 aa, 46 kDa
观测分子量 40 kDa
GenBank蛋白编号BC007363
基因名称 BCKDK
Gene ID (NCBI) 10295
RRIDAB_2878174
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14874
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BCKDK antibody 15718-1-APDownload protocol
IHC protocol for BCKDK antibody 15718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.

Authors - Yang Yang
View Article
mouseWB

Biochim Biophys Acta Mol Basis Dis

Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury

Authors - Xiao Han
View Article