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BCKDK Polyclonal antibody

BCKDK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15718-1-AP

Print datasheet

Synonyms

BCKDK

验证数据展示

  • WB analysis using 15718-1-AP

    WB analysis using 15718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 15718-1-AP

    IHC staining of mouse pancreas using 15718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCKDK antibody included
  • IHC staining of mouse pancreas using 15718-1-AP

    IHC staining of mouse pancreas using 15718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCKDK antibody included

经过测试的应用

Positive WB detected inmouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BCKDK Ready-To-Use IHC Kit. BCKDK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15718-1-AP targets BCKDK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen BCKDK fusion protein Ag8395 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name branched chain ketoacid dehydrogenase kinase
Synonyms BCKDK
Calculated Molecular Weight 412 aa, 46 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC007363
Gene Symbol BCKDK
Gene ID (NCBI) 10295
RRIDAB_2878174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BCKDK antibody 15718-1-APDownload protocol
IHC protocol for BCKDK antibody 15718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.

Authors - Yang Yang
View Article
mouseWB

Biochim Biophys Acta Mol Basis Dis

Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury

Authors - Xiao Han
View Article