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BCL2L13 Polyclonal antibody

BCL2L13 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16612-1-AP

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Synonyms

BCL2L13, MIL1, Protein Mil1



经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, HepG2 cells, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16612-1-AP targets BCL2L13 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen BCL2L13 fusion protein Ag9841 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-like 13 (apoptosis facilitator)
Synonyms BCL2L13, MIL1, Protein Mil1
Calculated Molecular Weight 485 aa, 53 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC003032
Gene Symbol BCL2L13
Gene ID (NCBI) 23786
RRIDAB_1850928
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L13/MIL1 gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. BLC2L13 gene is highest expressed in heart, placenta and pancreas. The encoded protein Bcl-rambo may promote the activation of caspase-3 and apoptosis. The Bcl-rambo cell death activity was induced by its membrane-anchored C-terminal domain and not by the Bcl-2 homology region (PMID: 11262395). Multivariate analysis demonstrated that high expression of BCL2L13 was an independent prognostic factor for in childhood acute lymphoblastic leukemia (PMID: 20109966).

实验方案

Product Specific Protocols
WB protocol for BCL2L13 antibody 16612-1-APDownload protocol
IHC protocol for BCL2L13 antibody 16612-1-APDownload protocol
IP protocol for BCL2L13 antibody 16612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Cancer Commun (Lond)

Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites

Authors - Ji Eon Kim
humanWB

Nat Commun

Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation.

Authors - Tomokazu Murakawa
human,mouseWB

Autophagy

BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.

Authors - Xiaoli Wu
mouseWB

Autophagy

BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.

Authors - Simone C da Silva Rosa
humanWB,IHC,IF

Proc Natl Acad Sci U S A

Bcl2L13 is a ceramide synthase inhibitor in glioblastoma.

Authors - Samuel A Jensen
  • KD Validated
mouse,ratIF

Glia

Neuroglial transmitophagy and Parkinson's disease.

Authors - Ingrid Morales