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BCL2L14 Polyclonal antibody

BCL2L14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 12846-1-AP

Print datasheet

Synonyms

Bcl2-L-14, Apoptosis regulator Bcl-G, Apoptosis regulator Bcl G, Apoptosis facilitator Bcl-2-like protein 14

验证数据展示

  • WB analysis of A549 using 12846-1-AP

    WB analysis of A549 using 12846-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12846-1-AP (BCL2L14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12846-1-AP

    IHC staining of human prostate cancer using 12846-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12846-1-AP (BCL2L14 antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of MCF-7 using 12846-1-AP

    IF Staining of MCF-7 using 12846-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12846-1-AP (BCL2L14 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Jurkat using 12846-1-AP

    FC experiment of Jurkat using 12846-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug BCL2L14 Polyclonal antibody (12846-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of human lung using 12846-1-AP

    WB analysis of human lung using 12846-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12846-1-AP (BCL2L14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, human lung tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12846-1-AP targets BCL2L14 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen BCL2L14 fusion protein Ag3926 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-like 14 (apoptosis facilitator)
Synonyms Bcl2-L-14, Apoptosis regulator Bcl-G, Apoptosis regulator Bcl G, Apoptosis facilitator Bcl-2-like protein 14
Calculated Molecular Weight 327 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC025778
Gene Symbol BCL2L14
Gene ID (NCBI) 79370
RRIDAB_2290479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZR8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BCL2L14 antibody 12846-1-APDownload protocol
IHC protocol for BCL2L14 antibody 12846-1-APDownload protocol
IF protocol for BCL2L14 antibody 12846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols