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BCL6 Monoclonal antibody, PBS Only

BCL6 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E6B1

Cat no : 66340-1-PBS

Print datasheet

Synonyms

BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein

验证数据展示

  • WB analysis of Daudi using 66340-1-Ig (same clone as 66340-1-PBS)

    WB analysis of Daudi using 66340-1-Ig (same clone as 66340-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

    IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 Antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH9). The signal was enhanced by soaking the slide in 1% CuSO4 for 10 minutes after DAB development. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

    IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer (pH9).The signal was enhanced by soaking the slide in 1% CuSO4 for 10 minutes after DAB development. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

产品信息

66340-1-PBS targets BCL6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen BCL6 fusion protein Ag15519 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name B-cell CLL/lymphoma 6
Synonyms BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein
Calculated Molecular Weight 706 aa, 79 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC150184
Gene Symbol BCL6
Gene ID (NCBI) 604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41182
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.