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BCL9 Recombinant antibody

BCL9 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240682A9

Cat no : 83760-3-RR

Print datasheet

Synonyms

Bcl-9, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein, B cell CLL/lymphoma 9

验证数据展示

  • IF Staining of HeLa using 83760-3-RR

    IF Staining of HeLa using 83760-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL9 antibody (83760-3-RR, Clone: 240682A9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of SH-SY5Y using 83760-3-RR

    FC experiment of SH-SY5Y using 83760-3-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Bcl9 Recombinant Antibody (83760-3-RR, Clone:240682A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83760-3-RR

    Affinity and Kinetic Characterization of 83760-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83760-3-RR against Human BCL9 were performed. The affinity constant is 5.39 nM.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83760-3-RR is BCL9 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen BCL9 fusion protein Ag19158 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B-cell CLL/lymphoma 9
Synonyms Bcl-9, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein, B cell CLL/lymphoma 9
Calculated Molecular Weight 1426 aa, 149 kDa
GenBank Accession NumberBC116451
Gene Symbol BCL9
Gene ID (NCBI) 607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00512
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

实验方案

Product Specific Protocols
IF protocol for BCL9 antibody 83760-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols