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BCLAF1 Monoclonal antibody, PBS Only

BCLAF1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6G7

Cat no : 67860-1-PBS

Print datasheet

Synonyms

KIAA0164, Btf, BCLAF1 and THRAP3 family member 1, Bcl-2-associated transcription factor 1, 1D6G7

验证数据展示

  • WB analysis using 67860-1-Ig (same clone as 67860-1-PBS)

    WB analysis using 67860-1-Ig (same clone as 67860-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67860-1-Ig (BCLAF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67860-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67860-1-Ig (same clone as 67860-1-PBS)

    IHC staining of mouse testis using 67860-1-Ig (same clone as 67860-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67860-1-Ig (BCLAF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67860-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCLAF1 antibody included
  • IHC staining of mouse testis using 67860-1-Ig (same clone as 67860-1-PBS)

    IHC staining of mouse testis using 67860-1-Ig (same clone as 67860-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67860-1-Ig (BCLAF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67860-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCLAF1 antibody included
  • IF Staining of HepG2 using 67860-1-Ig (same clone as 67860-1-PBS)

    IF Staining of HepG2 using 67860-1-Ig (same clone as 67860-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCLAF1 antibody (67860-1-Ig, Clone: 1D6G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67860-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67860-1-Ig (same clone as 67860-1-PBS)

    FC experiment of HepG2 using 67860-1-Ig (same clone as 67860-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human BCLAF1 (67860-1-Ig, Clone:1D6G7) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67860-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy BCLAF1 Ready-To-Use IHC Kit. BCLAF1 primary antibody included.

产品信息

67860-1-PBS targets BCLAF1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BCLAF1 fusion protein Ag25210 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BCL2-associated transcription factor 1
Synonyms KIAA0164, Btf, BCLAF1 and THRAP3 family member 1, Bcl-2-associated transcription factor 1, 1D6G7
Calculated Molecular Weight 920 aa, 106 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberBC132780
Gene Symbol BCLAF1
Gene ID (NCBI) 9774
RRIDAB_2918618
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NYF8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.