验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12107-1-AP targets BCOR in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IP, CoIP, ChIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | BCOR fusion protein Ag2752 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | BCL6 co-repressor |
别名 | ANOP2, BCOR, KIAA1575, MAA2, MCOPS2 |
计算分子量 | 1703 aa, 186 kDa |
观测分子量 | 186 kDa |
GenBank蛋白编号 | BC009675 |
基因名称 | BCOR |
Gene ID (NCBI) | 54880 |
RRID | AB_2290335 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q6W2J9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCOR, a corepressor of BCL6, binds to transcriptional factors and specifically inhibits gene expression. BCOR plays a key role in the early embryonic development, mesenchymal stem cell function and hematopiesis. BCOR can associate with HDACs, the polycomb group protein, polycomb group Ring finger protein1/nervous system polycomb1 (PCGF1/NSPC1), histone demethylase, F-box- and leucine-rich repeat protein10(FBXL10). Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem
实验方案
Product Specific Protocols | |
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WB protocol for BCOR antibody 12107-1-AP | Download protocol |
IHC protocol for BCOR antibody 12107-1-AP | Download protocol |
IP protocol for BCOR antibody 12107-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Genet Consistent in-frame internal tandem duplications of BCOR characterize clear cell sarcoma of the kidney. | ||
Blood Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia | ||
Nucleic Acids Res Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis. | ||
Int J Mol Sci BCOR Internal Tandem Duplication Expression in Neural Stem Cells Promotes Growth, Invasion, and Expression of PRC2 Targets. | ||
Sci Rep Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells. | ||
Cell Prolif Secreted frizzled-related protein 2 promotes the osteo/odontogenic differentiation and paracrine potentials of stem cells from apical papilla under inflammation and hypoxia conditions. |