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验证数据展示

  • WB analysis of SW480 using 68611-1-Ig

    WB analysis of SW480 using 68611-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68611-1-Ig

    WB analysis using 68611-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 68611-1-Ig

    WB analysis of RAW 264.7 using 68611-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 68611-1-Ig

    WB analysis of HEK-293 using 68611-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

BCOR Monoclonal antibody

BCOR Monoclonal Antibody for WB, ELISA
Cat No. 68611-1-Ig

Print datasheet

CloneNo. 1F12H6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

ANOP2, BCOR, FLJ20285, FLJ38041, KIAA1575, MAA2, MCOPS2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, SW480 cells, HEK-293 cells, RAW 264.7 cells, MCF-7 cells, Jurkat cells, MOLT-4 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68611-1-Ig targets BCOR in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen BCOR fusion protein Ag33785 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BCL6 co-repressor
Synonyms ANOP2, BCOR, FLJ20285, FLJ38041, KIAA1575, MAA2, MCOPS2
Calculated Molecular Weight 1703 aa, 186 kDa
Observed Molecular Weight186-200 kDa
GenBank Accession NumberBC009675
Gene Symbol BCOR
Gene ID (NCBI) 54880
RRIDAB_3085306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6W2J9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCOR, a corepressor of BCL6, binds to transcriptional factors and specifically inhibits gene expression. BCOR plays a key role in the early embryonic development, mesenchymal stem cell function and hematopiesis. BCOR can associate with HDACs, the polycomb group protein, polycomb group Ring finger protein1/nervous system polycomb1 (PCGF1/NSPC1), histone demethylase, F-box- and leucine-rich repeat protein10(FBXL10). Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem.

实验方案

Product Specific Protocols
WB protocol for BCOR antibody 68611-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols