验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, COS-7 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy BCR Ready-To-Use IHC Kit. BCR primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
22585-1-AP targets BCR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, monkey |
| 文献引用反应性 | human, mouse |
| 免疫原 | BCR fusion protein Ag18281 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | breakpoint cluster region |
| 别名 | Renal carcinoma antigen NY-REN-26, EC:2.7.11.1, breakpoint cluster region, BCR1, BCR 1 |
| 计算分子量 | 143 kDa |
| 观测分子量 | 150-160 kDa |
| GenBank蛋白编号 | BC066122 |
| 基因名称 | BCR |
| Gene ID (NCBI) | 613 |
| RRID | AB_2879127 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P11274 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BCR antibody 22585-1-AP | Download protocol |
| IHC protocol for BCR antibody 22585-1-AP | Download protocol |
| IF protocol for BCR antibody 22585-1-AP | Download protocol |
| IP protocol for BCR antibody 22585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neurogastroenterol Motil Loss function of Bcr mutation causes gastrointestinal dysmotility and brain developmental defects.
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