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验证数据展示

  • WB analysis of HEK-293 using 60212-1-Ig

    WB analysis of HEK-293 using 60212-1-Ig

    WB result of BCS1L antibody (60212-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCS1L transfected HEK-293 cells.

  • WB analysis of COLO 320 using 60212-1-Ig

    WB analysis of COLO 320 using 60212-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60212-1-Ig (BCS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 60212-1-Ig

    IHC staining of human gliomas using 60212-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human gliomas using 60212-1-Ig

    IHC staining of human gliomas using 60212-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 60212-1-Ig

    IF Staining of MCF-7 using 60212-1-Ig

    Immunofluorescent analysis of () fixed MCF-7 cells using 60212-1-Ig (BCS1L antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • Featured Product
  • KD/KO验证

BCS1L Monoclonal antibody

BCS1L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60212-1-Ig

产品说明书

CloneNo. 3G6H1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

BCS1L, BJS, FLNMS, GRACILE, h BCS, h BCS1, Hs.6719, Mitochondrial chaperone BCS1, PTD

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inColo320 cells, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman gliomas tissue, human brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60212-1-Ig targets BCS1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 BCS1L fusion protein Ag18280 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 BCS1-like (yeast)
别名 BCS1L, BJS, FLNMS, GRACILE, h BCS, h BCS1, Hs.6719, Mitochondrial chaperone BCS1, PTD
计算分子量 48 kDa
观测分子量 47 kDa
GenBank蛋白编号BC007500
基因名称 BCS1L
Gene ID (NCBI) 617
RRIDAB_11042318
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y276
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human BCS1-like (BCS1L), a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Recently studies indicated that the mutations in this protein may cause the GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome, a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism.

实验方案

Product Specific Protocols
WB protocol for BCS1L antibody 60212-1-IgDownload protocol
IHC protocol for BCS1L antibody 60212-1-IgDownload protocol
IF protocol for BCS1L antibody 60212-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols