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验证数据展示

  • WB analysis using 68279-1-Ig (same clone as 68279-1-PBS)

    WB analysis using 68279-1-Ig (same clone as 68279-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68279-1-Ig (BDH2 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68279-1-Ig (same clone as 68279-1-PBS)

    WB analysis of HeLa using 68279-1-Ig (same clone as 68279-1-PBS)

    WB result of BDH2 antibody (68279-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells. This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68279-1-Ig (same clone as 68279-1-PBS)

    IF Staining of HepG2 using 68279-1-Ig (same clone as 68279-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BDH2 antibody (68279-1-Ig, Clone: 1B6H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BDH2 Monoclonal antibody, PBS Only

BDH2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68279-1-PBS

Print datasheet

CloneNo. 1B6H5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

BDH2, DHRS6, EFA6R, FLJ13261, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

68279-1-PBS targets BDH2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen BDH2 fusion protein Ag26108 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 3-hydroxybutyrate dehydrogenase, type 2
Synonyms BDH2, DHRS6, EFA6R, FLJ13261, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC095414
Gene Symbol BDH2
Gene ID (NCBI) 56898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BUT1
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytosolic type 2-hydroxybutyrate dehydrogenase, BDH2, originally named DHRS6, plays a physiological role in the cytosolic utilization of ketone bodies, which can subsequently enter mitochondria and the tricarboxylic acid cycle (PMID: 16380372). Moreover, BDH2 catalyzes the production of 2, 3-DHBA during enterobactin biosynthesis and participates in 24p3 (LCN2)-mediated iron transport and apoptosis (PMID: 23150981). It has 3 isoforms with molecular weights of 27, 19, and 16 kDa.