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BDNF Polyclonal antibody

BDNF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 25699-1-AP

Print datasheet

Synonyms

Abrineurin, BDNF



经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25699-1-AP targets BDNF in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, macaque
Immunogen BDNF fusion protein Ag22489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name brain-derived neurotrophic factor
Synonyms Abrineurin, BDNF
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC029795
Gene Symbol BDNF
Gene ID (NCBI) 627
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23560
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

实验方案

Product Specific Protocols
WB protocol for BDNF antibody 25699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Neurosci

Early BDNF Treatment Ameliorates Cell Loss in the Entorhinal Cortex of APP Transgenic Mice.

Authors - Nagahara Alan H AH
mouseWB

Neuropsychopharmacology

CPEB3-dowregulated Nr3c1 mRNA translation confers resilience to developing posttraumatic stress disorder-like behavior in fear-conditioned mice.

Authors - Wen-Hsin Lu
mouseWB

Cell Mol Life Sci

Furin promotes dendritic morphogenesis and learning and memory in transgenic mice.

Authors - Binglin Zhu
IHC

J Neurotrauma

Bone Marrow Stromal Cell Intraspinal Transplants Fail to Improve Motor Outcomes in a Severe Model of Spinal Cord Injury.

Authors - John H Brock
mouseWB

Exp Neurol

Neuroprotective and neuro-rehabilitative effects of acute purinergic receptor P2X4 (P2X4R) blockade after ischemic stroke.

Authors - Pranay Srivastava
mouseWB

Front Pharmacol

Danshen-Chuanxiong-Honghua Ameliorates Cerebral Impairment and Improves Spatial Cognitive Deficits after Transient Focal Ischemia and Identification of Active Compounds.

Authors - Xianhua Zhang