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BEGAIN Polyclonal antibody

BEGAIN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14956-1-AP

Print datasheet

Synonyms

BEGAIN, KIAA1446

验证数据展示

  • WB analysis using 14956-1-AP

    WB analysis using 14956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14956-1-AP (BEGAIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14956-1-AP

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 14956-1-AP

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, JAR cells, SH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue, mouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14956-1-AP targets BEGAIN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen BEGAIN fusion protein Ag6794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name brain-enriched guanylate kinase-associated homolog (rat)
Synonyms BEGAIN, KIAA1446
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC002607
Gene Symbol BEGAIN
Gene ID (NCBI) 57596
RRIDAB_2064487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUH8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BEGAIN antibody 14956-1-APDownload protocol
IHC protocol for BEGAIN antibody 14956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS One

The First Nationwide Survey and Genetic Analyses of Bardet-Biedl Syndrome in Japan.

Authors - Makito Hirano
View Article