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BEND4 Polyclonal antibody

BEND4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24711-1-AP

Print datasheet

Synonyms

BEN domain containing 4, BEND4, CCDC4

验证数据展示

  • WB analysis of mouse testis using 24711-1-AP

    WB analysis of mouse testis using 24711-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24711-1-AP (BEND4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 24711-1-AP

    IHC staining of human heart using 24711-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon cancer using 24711-1-AP

    IHC staining of human colon cancer using 24711-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24711-1-AP targets BEND4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen BEND4 fusion protein Ag20169 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BEN domain containing 4
Synonyms BEN domain containing 4, BEND4, CCDC4
Calculated Molecular Weight 534 aa, 58 kDa
Observed Molecular Weight 55-58 kDa, 33-36 kDa
GenBank Accession NumberBC128592
Gene Symbol BEND4
Gene ID (NCBI) 389206
RRIDAB_2879684
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZU67
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BEND4, also named as CCDC4, has 5 isoforms with MW 55-58 kDa, 45-48 kDa and 36 kDa.

实验方案

Product Specific Protocols
WB protocol for BEND4 antibody 24711-1-APDownload protocol
IHC protocol for BEND4 antibody 24711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols