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Bestrophin-3 Polyclonal antibody

Bestrophin-3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20443-1-AP

Print datasheet

Synonyms

BEST3, Bestrophin 3, Bestrophin-3, VMD2L3

验证数据展示

  • WB analysis of mouse colon using 20443-1-AP

    WB analysis of mouse colon using 20443-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 20443-1-AP (Bestrophin-3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 20443-1-AP

    IHC staining of human skeletal muscle using 20443-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20443-1-AP (Bestrophin-3 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 20443-1-AP

    IHC staining of human skeletal muscle using 20443-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20443-1-AP (Bestrophin-3 antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 20443-1-AP is Bestrophin-3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Bestrophin-3 fusion protein Ag14255 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bestrophin 3
Synonyms BEST3, Bestrophin 3, Bestrophin-3, VMD2L3
Calculated Molecular Weight 668 aa, 76 kDa
Observed Molecular Weight 76-78 kDa, 50-55 kDa
GenBank Accession NumberBC028087
Gene Symbol BEST3
Gene ID (NCBI) 144453
RRIDAB_10666435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bestrophin-3 (BEST3) is a member of the Bestrophin (VMD2) Ca2+-activated Cl channel gene family. Bestrophin-3 is involved in the regulation of cell proliferation, apoptosis and differentiation of a variety of physiological functions (PMID: 25329324). Human BEST3 gene is located at chromosome 12q15. The expression of BEST3 was predominantly observed in skeletal muscle and weaker in brain, spinal cord, bone marrow, retina, thymus, and testis (PMID: 12032738).

实验方案

Product Specific Protocols
WB protocol for Bestrophin-3 antibody 20443-1-APDownload protocol
IHC protocol for Bestrophin-3 antibody 20443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Rep

A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum.

Authors - Lichang Wu
View Article
WB

J Mol Cell Cardiol

Sarcolemmal Ca(2+)-entry through L-type Ca(2+) channels controls the profile of Ca(2+)-activated Cl(-) current in canine ventricular myocytes.

Authors - Balázs Horváth
View Article