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验证数据展示

  • WB analysis of mouse colon using 20443-1-AP

    WB analysis of mouse colon using 20443-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 20443-1-AP (Bestrophin-3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 20443-1-AP

    IHC staining of human skeletal muscle using 20443-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20443-1-AP (Bestrophin-3 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 20443-1-AP

    IHC staining of human skeletal muscle using 20443-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20443-1-AP (Bestrophin-3 antibody at dilution of 1:200 (under 40x lens).

Bestrophin-3 Polyclonal antibody

Bestrophin-3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20443-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

BEST3, Bestrophin 3, Bestrophin-3, VMD2L3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20443-1-AP targets Bestrophin-3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 Bestrophin-3 fusion protein Ag14255 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bestrophin 3
别名 BEST3, Bestrophin 3, Bestrophin-3, VMD2L3
计算分子量 668 aa, 76 kDa
观测分子量 76-78 kDa, 50-55 kDa
GenBank蛋白编号BC028087
基因名称 BEST3
Gene ID (NCBI) 144453
RRIDAB_10666435
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N1M1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bestrophin-3 (BEST3) is a member of the Bestrophin (VMD2) Ca2+-activated Cl channel gene family. Bestrophin-3 is involved in the regulation of cell proliferation, apoptosis and differentiation of a variety of physiological functions (PMID: 25329324). Human BEST3 gene is located at chromosome 12q15. The expression of BEST3 was predominantly observed in skeletal muscle and weaker in brain, spinal cord, bone marrow, retina, thymus, and testis (PMID: 12032738).

实验方案

Product Specific Protocols
WB protocol for Bestrophin-3 antibody 20443-1-APDownload protocol
IHC protocol for Bestrophin-3 antibody 20443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Rep

A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum.

Authors - Lichang Wu
View Article
WB

J Mol Cell Cardiol

Sarcolemmal Ca(2+)-entry through L-type Ca(2+) channels controls the profile of Ca(2+)-activated Cl(-) current in canine ventricular myocytes.

Authors - Balázs Horváth
View Article