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验证数据展示

  • WB analysis using 11794-1-AP

    WB analysis using 11794-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11794-1-AP (BET1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11794-1-AP

    IHC staining of human colon cancer using 11794-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11794-1-AP (BET1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BET1 Polyclonal antibody

BET1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11794-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

hBET1, Golgi vesicular membrane-trafficking protein p18, BET1 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, U-87 MG cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11794-1-AP targets BET1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 BET1 fusion protein Ag2261 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 blocked early in transport 1 homolog (S. cerevisiae)
别名 hBET1, Golgi vesicular membrane-trafficking protein p18, BET1 homolog
计算分子量 13 kDa
观测分子量13 kDa
GenBank蛋白编号BC000899
基因名称 BET1
Gene ID (NCBI) 10282
RRIDAB_3085381
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15155
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BET1 antibody 11794-1-APDownload protocol
IHC protocol for BET1 antibody 11794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols