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Beta ENaC Polyclonal antibody

Beta ENaC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14134-1-AP

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Synonyms

SCNN1B, ENaCbeta, ENaCb, Beta-NaCH, Beta-ENaC



经过测试的应用

Positive WB detected inPC-3 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14134-1-AP targets Beta ENaC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, hamster
Immunogen Beta ENaC fusion protein Ag5348 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sodium channel, nonvoltage-gated 1, beta
Synonyms SCNN1B, ENaCbeta, ENaCb, Beta-NaCH, Beta-ENaC
Calculated Molecular Weight 640 aa, 73 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC036352
Gene Symbol Beta ENaC
Gene ID (NCBI) 6338
RRIDAB_2184378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51168
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCNN1B, also named as SCNEB, ENaCB, Beta-NaCH and Beta-ENaC, belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family and SCNN1B subfamily. Sodium permeable non-voltage-sensitive ion channel is inhibited by the diuretic amiloride. SCNN1B mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. It controls the reabsorption of sodium in kidney, colon, lung and sweat glands. SCNN1B plays a role in taste perception.

实验方案

Product Specific Protocols
WB protocol for Beta ENaC antibody 14134-1-APDownload protocol
IHC protocol for Beta ENaC antibody 14134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Exp Med

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Authors - Yalong Wang
pigWB

Anim Nutr

Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function.

Authors - Liuqin He
mouseWB

Front Pharmacol

Melatonin Attenuates Sepsis-Induced Acute Lung Injury Through Improvement of Epithelial Sodium Channel-Mediated Alveolar Fluid Clearance Via Activation of SIRT1/SGK1/Nedd4-2 Signaling Pathway.

Authors - Jing Li
ratWB

J Neurotrauma

Activity-based training reverses SCI-induced changes in kidney receptor densities and membrane proteins.

Authors - Jason H Gumbel
hamsterWB

Exp Cell Res

Calreticulin positively regulates the expression and function of epithelial sodium channel.

Authors - Sugahara Takuya T
  • KD Validated
humanWB

Toxins (Basel)

Listeriolysin O Causes ENaC Dysfunction in Human Airway Epithelial Cells.

Authors - Guang Yang