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验证数据展示

  • IHC staining of human osteosarcoma using 16157-1-AP

    IHC staining of human osteosarcoma using 16157-1-AP

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human osteosarcoma using 16157-1-AP

    IHC staining of human osteosarcoma using 16157-1-AP

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat lung using 16157-1-AP

    IHC staining of rat lung using 16157-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 16157-1-AP

    IHC staining of mouse lung using 16157-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U2OS using 16157-1-AP

    FC experiment of U2OS using 16157-1-AP

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug Osteocalcin/OCN Polyclonal antibody (16157-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of NIH/3T3 using 16157-1-AP

    IF Staining of NIH/3T3 using 16157-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Osteocalcin antibody (16157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Osteocalcin/OCN Polyclonal antibody

Osteocalcin/OCN Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 16157-1-AP

产品说明书

引用文献(7)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, FC (Intra), ELISA and More (2)

别名

BGLAP, Osteocalcin, Gamma-carboxyglutamic acid-containing protein, Bone Gla protein, BGP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman osteosarcoma tissue, mouse lung tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16157-1-AP targets Osteocalcin/OCN in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone gamma-carboxyglutamate (gla) protein
别名 BGLAP, Osteocalcin, Gamma-carboxyglutamic acid-containing protein, Bone Gla protein, BGP
计算分子量 11 kDa
GenBank蛋白编号NM_199173
基因名称 Osteocalcin
Gene ID (NCBI) 632
RRIDAB_2878225
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02818
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Osteocalcin/OCN antibody 16157-1-APDownload protocol
IF protocol for Osteocalcin/OCN antibody 16157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Aging (Albany NY)

Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.

Authors - Jie Xu
View Article
mouseIHC

Front Pharmacol

Naringenin is a Potential Anabolic Treatment for Bone Loss by Modulating Osteogenesis, Osteoclastogenesis, and Macrophage Polarization.

Authors - Xin Zhou
View Article
mouseIHC

Bone

Macrophage migration inhibitory factor promotes heterotopic ossification by mediating ROS/HIF-1α positive feedback loop and activating Wnt/β-catenin signaling pathway

Authors - Ping Li
View Article
ratIF

Med Oncol

CRP inhibits the osteoblastic differentiation of OPCs via the up-regulation of primary cilia and repression of the Hedgehog signaling pathway

Authors - Jie Xu
View Article
mouseWB

Aging Dis

Hypermethylation of Bmp2 and Fgfr2 Promoter Regions in Bone Marrow Mesenchymal Stem Cells Leads to Bone Loss in Prematurely Aged Mice

Authors - Yao Wang
View Article
ratIF

Cell Rep Med

Bone transport induces the release of factors with multi-tissue regenerative potential for diabetic wound healing in rats and patients

Authors - Jing Xie
View Article