验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells, HepG2 cells, mouse skeletal muscle tissue, pig cartilage tissue |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human kidney tissue, human liver tissue, human liver cancer tissue, human lung tissue, human renal cell carcinoma tissue, human stomach cancer tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16409-1-AP targets Biglycan in WB, IHC, IF/ICC, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, Cell treatment |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, canine |
| 免疫原 | Biglycan fusion protein Ag0683 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | biglycan |
| 别名 | BGN, PRG1, PG-S1, PGI, PG S1 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 40-48 kDa |
| GenBank蛋白编号 | BC004244 |
| 基因名称 | Biglycan |
| Gene ID (NCBI) | 633 |
| RRID | AB_10804656 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21810 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Biglycan antibody 16409-1-AP | Download protocol |
| IHC protocol for Biglycan antibody 16409-1-AP | Download protocol |
| IF protocol for Biglycan antibody 16409-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncoimmunology Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer. | ||
Cancers (Basel) Biglycan Promotes Cancer Stem Cell Properties, NFκB Signaling and Metastatic Potential in Breast Cancer Cells.
| ||
J Biol Eng Proteomic analysis of decellularized mice liver and kidney extracellular matrices | ||
Oncoimmunology Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells. | ||
Cell Prolif Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway. | ||
Cancer Med Establishment of a risk model by integrating hypoxia genes in predicting prognosis of esophageal squamous cell carcinoma |
