• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of SH-SY5Y using 25622-1-AP

    WB analysis of SH-SY5Y using 25622-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25622-1-AP (BHLHE22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25622-1-AP

    IHC staining of human brain using 25622-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25622-1-AP (BHLHE22 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of SH-SY5Y using 25622-1-AP

    IF Staining of SH-SY5Y using 25622-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 25622-1-AP (BHLHE22 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

BHLHE22 Polyclonal antibody

BHLHE22 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25622-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

Beta3, BHLHB5, BHLHE22, CAGL85, TNRC20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25622-1-AP targets BHLHE22 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse, goat
免疫原 BHLHE22 fusion protein Ag20966 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic helix-loop-helix family, member e22
别名 Beta3, BHLHB5, BHLHE22, CAGL85, TNRC20
计算分子量 381 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC156671
基因名称 BHLHE22
Gene ID (NCBI) 27319
RRIDAB_2880165
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFJ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Basic helix-loop-helix family member e22 (BHLHE22), a basic helix loop helix transcription factor family member, acts as a transcriptional repressor and has a role in cell differentiation in neuron development (PMID: 36941015). BHLHE22 expression serves as a potential biomarker for ICI treatment prediction and favorable prognosis, and that is associated with microenvironment immune status (PMID: 35806162).

实验方案

Product Specific Protocols
WB protocol for BHLHE22 antibody 25622-1-APDownload protocol
IHC protocol for BHLHE22 antibody 25622-1-APDownload protocol
IF protocol for BHLHE22 antibody 25622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Deubiquitinase OTUD3 regulates metabolism homeostasis in response to nutritional stresses.

Authors - Na Zhou
View Article
goatWB

PLoS One

Chi-miR-4110 promotes granulosa cell apoptosis by targeting Sma- and Mad-related protein 2 (Smad2) in the caprine ovary.

Authors - Xiaopeng An
View Article