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BHMT2 Polyclonal antibody

BHMT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16351-1-AP

Print datasheet

Synonyms

BHMT2

验证数据展示

  • IHC staining of human kidney using 16351-1-AP

    IHC staining of human kidney using 16351-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16351-1-AP (BHMT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BHMT2 antibody included
  • WB analysis of mouse liver using 16351-1-AP

    WB analysis of mouse liver using 16351-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16351-1-AP (BHMT2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BHMT2 Ready-To-Use IHC Kit. BHMT2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16351-1-AP targets BHMT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BHMT2 fusion protein Ag9558 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name betaine-homocysteine methyltransferase 2
Synonyms BHMT2
Calculated Molecular Weight 363 aa, 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC020665
Gene Symbol BHMT2
Gene ID (NCBI) 23743
RRIDAB_2878248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2M3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BHMT2 antibody 16351-1-APDownload protocol
IHC protocol for BHMT2 antibody 16351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols