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验证数据展示

  • WB analysis using 10188-1-AP

    WB analysis using 10188-1-AP

    Mouse eye tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10188-1-AP

    WB analysis of HeLa using 10188-1-AP

    WB result of TGFBI / BIGH3 antibody (10188-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBI / BIGH3 transfected HeLa cells.

  • IHC staining of human kidney using 10188-1-AP

    IHC staining of human kidney using 10188-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with TGFBI antibody included
  • IHC staining of human kidney using 10188-1-AP

    IHC staining of human kidney using 10188-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with TGFBI antibody included
  • IF Staining of A549 using 10188-1-AP

    IF Staining of A549 using 10188-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells, untreated (left) or TGF-β-treated (right), using TGFBI / BIGH3 antibody (10188-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Y79 using 10188-1-AP

    FC experiment of Y79 using 10188-1-AP

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug Anti-Human TGFBI / BIGH3 (10188-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of HeLa using 10188-1-AP

    IP experiment of HeLa using 10188-1-AP

    IP result of anti-TGFBI / BIGH3 (IP:10188-1-AP, 3ug; Detection:10188-1-AP 1:300) with HeLa cells lysate 1000ug.

  • Featured Product
  • KD/KO Validated

TGFBI/BIGH3 Polyclonal antibody

TGFBI/BIGH3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10188-1-AP
Publications(70)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Synonyms

TGFBI, TGFBI / BIGH3, RGD-containing collagen-associated protein, RGD-CAP, Kerato-epithelin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue, mouse liver tissue, human kidney tissue, Y79 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, mouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTGF beta 1 treated A549 cells, Y79 cells
Positive FC (Intra) detected inY79 cells
Planning an IHC experiment? We recommend our IHCeasy TGFBI Ready-To-Use IHC Kit. TGFBI primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10188-1-AP targets TGFBI/BIGH3 in WB, IHC, IF/ICC, FC (Intra), IP, Neutralization, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, Neutralization, Cell treatment
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen TGFBI/BIGH3 fusion protein Ag0241 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transforming growth factor, beta-induced, 68kDa
Synonyms TGFBI, TGFBI / BIGH3, RGD-containing collagen-associated protein, RGD-CAP, Kerato-epithelin
Calculated Molecular Weight 683 aa, 75 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC000097
Gene Symbol TGFBI
Gene ID (NCBI) 7045
ENSEMBL Gene IDENSG00000120708
RRIDAB_2202311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15582
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

实验方案

Product Specific Protocols
WB protocol for TGFBI/BIGH3 antibody 10188-1-APDownload protocol
IHC protocol for TGFBI/BIGH3 antibody 10188-1-APDownload protocol
IF protocol for TGFBI/BIGH3 antibody 10188-1-APDownload protocol
IP protocol for TGFBI/BIGH3 antibody 10188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Nat Cell Biol

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets.

Authors - Qin Zhang
View Article
humanWB

Nat Protoc

Comprehensive isolation of extracellular vesicles and nanoparticles

Authors - Qin Zhang
View Article
humanIP

Sci Adv

Acetylated K676 TGFBIp as a severity diagnostic blood biomarker for SARS-CoV-2 pneumonia.

Authors - Hee Ho Park
View Article
mouseWB,IHC

Genes Dev

Extracellular matrix protein betaig-h3/TGFBI promotes metastasis of colon cancer by enhancing cell extravasation.

Authors - Ma Chaoyu C
  • KD Validated
View Article
humanIHC,IF

Theranostics

Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2

Authors - Zirong Chen
View Article
humanWB,IP

Biomaterials

Dual peptide-dendrimer conjugate inhibits acetylation of transforming growth factor β-induced protein and improves survival in sepsis.

Authors - Wonhwa Lee
View Article