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  • Featured Product
  • KD/KO Validated

BIN1 Polyclonal antibody, PBS Only

BIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14647-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 14647-1-AP (same clone as 14647-1-PBS)

    WB analysis using 14647-1-AP (same clone as 14647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    IP experiment of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    IP result of anti-BIN1 (IP:14647-1-AP, 4ug; Detection:14647-1-AP 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

    IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BIN1 antibody included
  • IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

    IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BIN1 antibody included
  • IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy BIN1 Ready-To-Use IHC Kit. BIN1 primary antibody included.

产品信息

14647-1-PBS targets BIN1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BIN1 fusion protein Ag6240 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bridging integrator 1
Synonyms
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 50-65 kDa
GenBank Accession NumberBC004101
Gene Symbol BIN1
Gene ID (NCBI) 274
RRIDAB_2243396
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00499
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).