验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, U-937 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human spleen tissue, mouse heart tissue, human heart tissue, human skin tissue, human lung tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14245-1-AP targets BIN2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Immunogen | BIN2 fusion protein Ag5502 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | bridging integrator 2 |
| Synonyms | BIN2, BRAP 1, BRAP1, bridging integrator 2 |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 80-90 kDa |
| GenBank Accession Number | BC047686 |
| Gene Symbol | BIN2 |
| Gene ID (NCBI) | 51411 |
| RRID | AB_2063647 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBW5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BIN2 antibody 14245-1-AP | Download protocol |
| IHC protocol for BIN2 antibody 14245-1-AP | Download protocol |
| IP protocol for BIN2 antibody 14245-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
