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验证数据展示

  • WB analysis of Raji using 25432-1-AP

    WB analysis of Raji using 25432-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25432-1-AP (BIVM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 25432-1-AP

    IHC staining of human ovary using 25432-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25432-1-AP (BIVM Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 25432-1-AP

    IHC staining of human ovary using 25432-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25432-1-AP (BIVM Antibody) at dilution of 1:50 (under 40x lens).

BIVM Polyclonal antibody

BIVM Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25432-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

BIVM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25432-1-AP targets BIVM in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 BIVM fusion protein Ag22087 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic, immunoglobulin-like variable motif containing
别名 BIVM
计算分子量 503 aa, 57 kDa
观测分子量 32 kDa
GenBank蛋白编号BC075084
基因名称 BIVM
Gene ID (NCBI) 54841
RRIDAB_2880076
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UB2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BIVM has been identified using an electronic search based on the conservation of short sequence motifs within the variable region of immunoglobulin (Ig) genes. It is tightly linked (41 bp) and in the opposite transcriptional orientation to MGC5302 (also known as KDEL1 and EP58) in human. BIVM has two isoforms with MW 57 kDa and 32 kDa.

实验方案

Product Specific Protocols
WB protocol for BIVM antibody 25432-1-APDownload protocol
IHC protocol for BIVM antibody 25432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols