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Conjugate
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BLIMP1/PRDM1 Recombinant antibody

BLIMP1/PRDM1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242030B1

Cat no : 84625-3-RR

Print datasheet

Synonyms

BLIMP1, PRDM1, PRDI BF1, BLIMP 1, 242030B1

验证数据展示

  • WB analysis using 84625-3-RR

    WB analysis using 84625-3-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 84625-3-RR (BLIMP1/PRDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, A549 cell, A431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84625-3-RR targets BLIMP1/PRDM1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PR domain containing 1, with ZNF domain
Synonyms BLIMP1, PRDM1, PRDI BF1, BLIMP 1, 242030B1
Calculated Molecular Weight92 kDa
Observed Molecular Weight 96-110 kDa
GenBank Accession NumberNM_001198.3
Gene Symbol PRDM1
Gene ID (NCBI) 639
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75626-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BLIMP1(B lymphocyte-induced maturation protein 1), also known as PRDM1(PR domain zinc finger protein 1), is an evolutionarily conserved transcriptional regulator originally described as a repressor of gene transcription(PMID: 35154079). BLIMP1/PRDM1 is widely expressed in multiple cell types including B lymphocytes, T lymphocytes, mammary luminal progenitor cells and skin epithelial cells. BLIMP1/PRDM1 is a master regulator of terminal B cell differentiation and may also function as a tumor suppressor in the pathogenesis of DLBCL, where it is frequently inactivated by mutations and deletions.

实验方案

Product Specific Protocols
WB protocol for BLIMP1/PRDM1 antibody 84625-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols