验证数据展示
经过测试的应用
| Positive WB detected in | human colon tissue, mouse pancreas tissue, mouse spleen tissue |
| Positive IP detected in | mouse skin tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14941-1-AP targets BLMH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, hamster |
| 免疫原 | BLMH fusion protein Ag6888 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | bleomycin hydrolase |
| 别名 | EC:3.4.22.40, BMH, BLM hydrolase, bleomycin hydrolase, BH |
| 计算分子量 | 53 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC003616 |
| 基因名称 | BLMH |
| Gene ID (NCBI) | 642 |
| RRID | AB_2064803 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13867 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BLMH(Bleomycin hydrolase) is also named as BH, BMH and belongs to the peptidase C1 family. The gene is expressed in many human tissues with elevated expression levels found in testis, skeletal muscle, and pancreas, and low levels of expression in colon and peripheral blood leukocytes(PMID:8639621). The protein catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. BH-immunoreactivity is essentially located in the cytoplasm and at the outer membrane of the rough endoplasmic reticulum of epithelial cells of the kidney, as well as in that of hepatocytes of the liver(PMID:17158865).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BLMH antibody 14941-1-AP | Download protocol |
| IHC protocol for BLMH antibody 14941-1-AP | Download protocol |
| IF protocol for BLMH antibody 14941-1-AP | Download protocol |
| IP protocol for BLMH antibody 14941-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Mol Biol DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblasts. | ||
Int J Oncol N6-methyladenosine-induced long non-coding RNA PVT1 regulates the miR-27b-3p/BLM axis to promote prostate cancer progression | ||
J Dermatol Sci Increased production of natural moisturizing factors and bleomycin hydrolase activity in elderly human skin | ||
Cancer Gene Ther METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1 |
