验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19687-1-AP targets BLOC1S1-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | biogenesis of lysosomal organelles complex-1, subunit 1 |
| 别名 | BLOC 1 subunit 1, BLOC1S1, BLOC1S1-Specific, BLOS1, GCN5 like protein 1, GCN5L1, MICoA, Protein RT14, RT14 |
| 计算分子量 | 17 kDa |
| 观测分子量 | 14-17 kDa |
| GenBank蛋白编号 | NM_001487 |
| 基因名称 | BLOC1S1 |
| Gene ID (NCBI) | 2647 |
| RRID | AB_10666440 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78537 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BLOC1S1, also named as BLOS1, GCN5L1 and RT14, belongs to the BLOC1S1 family. BLOC1S1 may be involved in the biogenesis of specialized organelles of the endosomal-lysosomal system. The antibody is specific to NLOC1S1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BLOC1S1-Specific antibody 19687-1-AP | Download protocol |
| IHC protocol for BLOC1S1-Specific antibody 19687-1-AP | Download protocol |
| IP protocol for BLOC1S1-Specific antibody 19687-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α | ||
EMBO J Snapin promotes HIV-1 transmission from dendritic cells by dampening TLR8 signaling.
| ||
Autophagy HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons. | ||
Oxid Med Cell Longev General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease. | ||
FEBS J A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems. |
