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BLVRB Polyclonal antibody

BLVRB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17727-1-AP

Print datasheet

Synonyms

BVR-B, BVRB, BVR B, Biliverdin-IX beta-reductase, Biliverdin reductase B

验证数据展示

  • IHC staining of human liver using 17727-1-AP

    IHC staining of human liver using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 17727-1-AP

    WB analysis using 17727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IHC staining of rat kidney using 17727-1-AP

    IHC staining of rat kidney using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 17727-1-AP

    IHC staining of rat liver using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman liver tissue, rat kidney tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17727-1-AP targets BLVRB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen BLVRB fusion protein Ag12124 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name biliverdin reductase B (flavin reductase (NADPH))
Synonyms BVR-B, BVRB, BVR B, Biliverdin-IX beta-reductase, Biliverdin reductase B
Calculated Molecular Weight 206 aa, 22 kDa
Observed Molecular Weight 22-27 kDa
GenBank Accession NumberBC109371
Gene Symbol BLVRB
Gene ID (NCBI) 645
RRIDAB_2065087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30043
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BLVRB antibody 17727-1-APDownload protocol
IHC protocol for BLVRB antibody 17727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Bilirubin Oxidation End Products (BOXes) Induce Neuronal Oxidative Stress Involving the Nrf2 Pathway.

Authors - Yinzhong Lu
View Article