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验证数据展示

  • IHC staining of human liver using 17727-1-AP

    IHC staining of human liver using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 17727-1-AP

    WB analysis using 17727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IHC staining of rat kidney using 17727-1-AP

    IHC staining of rat kidney using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 17727-1-AP

    IHC staining of rat liver using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BLVRB Polyclonal antibody

BLVRB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17727-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

BVR-B, BVRB, BVR B, Biliverdin-IX beta-reductase, Biliverdin reductase B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman liver tissue, rat kidney tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17727-1-AP targets BLVRB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 BLVRB fusion protein Ag12124 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 biliverdin reductase B (flavin reductase (NADPH))
别名 BVR-B, BVRB, BVR B, Biliverdin-IX beta-reductase, Biliverdin reductase B
计算分子量 206 aa, 22 kDa
观测分子量 22-27 kDa
GenBank蛋白编号BC109371
基因名称 BLVRB
Gene ID (NCBI) 645
RRIDAB_2065087
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30043
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BLVRB antibody 17727-1-APDownload protocol
IHC protocol for BLVRB antibody 17727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Bilirubin Oxidation End Products (BOXes) Induce Neuronal Oxidative Stress Involving the Nrf2 Pathway.

Authors - Yinzhong Lu
View Article