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验证数据展示

  • WB analysis of Recombinant protein using 22188-1-AP

    WB analysis of Recombinant protein using 22188-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 22188-1-AP (BMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 22188-1-AP

    IHC staining of human liver cancer using 22188-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 22188-1-AP

    IHC staining of human liver cancer using 22188-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BMP3 Polyclonal antibody

BMP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22188-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

BMP 3, BMP 3A, BMP3, BMP-3, BMP3A, bone morphogenetic protein 3, Bone morphogenetic protein 3A, Osteogenin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

22188-1-AP targets BMP3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen BMP3 fusion protein Ag16750 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bone morphogenetic protein 3
Synonyms BMP 3, BMP 3A, BMP3, BMP-3, BMP3A, bone morphogenetic protein 3, Bone morphogenetic protein 3A, Osteogenin
Calculated Molecular Weight 472 aa, 53 kDa
GenBank Accession NumberBC096269
Gene Symbol BMP3
Gene ID (NCBI) 651
RRIDAB_2879019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12645
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. BMP3 plays an important role in embryonic development by inducing and patterning early skeletal formation. BMP3 has also been reported as a negative growth regulator in bone marrow progenitor cells through the inhibition of DNA synthesis and proliferation. Additionally, recent studies have revealed that BMP3 contributes to tumor development and progression.

实验方案

Product Specific Protocols
WB protocol for BMP3 antibody 22188-1-APDownload protocol
IHC protocol for BMP3 antibody 22188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Bone morphogenetic protein-3 is a negative regulator of transforming growth factor beta and fibrosis

Authors - Emma R Dorris
View Article
humanWB

Biol Trace Elem Res

Effects of Sodium Fluoride Treatment In Vitro on Cell Proliferation, BMP-2 and BMP-3 Expression in Human Osteosarcoma MG-63 Cells.

Authors - Yan Wei
View Article