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BMP5 Polyclonal antibody

BMP5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13253-1-AP

Print datasheet

Synonyms

BMP 5, BMP5, BMP-5, bone morphogenetic protein 5

验证数据展示

  • IHC staining of human colon using 13253-1-AP

    IHC staining of human colon using 13253-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 13253-1-AP

    IHC staining of human colon using 13253-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse lung using 13253-1-AP

    WB analysis of mouse lung using 13253-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse lung tissue, HUVEC cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13253-1-AP targets BMP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen BMP5 fusion protein Ag3952 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bone morphogenetic protein 5
Synonyms BMP 5, BMP5, BMP-5, bone morphogenetic protein 5
Calculated Molecular Weight 454 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC027958
Gene Symbol BMP5
Gene ID (NCBI) 653
RRIDAB_2877929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22003
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BMP5 antibody 13253-1-APDownload protocol
IHC protocol for BMP5 antibody 13253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

G9a Knockdown Suppresses Cancer Aggressiveness by Facilitating Smad Protein Phosphorylation through Increasing BMP5 Expression in Luminal A Type Breast Cancer.

Authors - Yunho Jin
View Article
humanWB

FASEB J

Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis

Authors - Xiang Gao
View Article
humanIHC

Mol Cancer

Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.

Authors - Erfei Chen
View Article