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BMPR1A Polyclonal antibody

BMPR1A Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 30608-1-AP

Print datasheet

Synonyms

ALK-3, ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3

验证数据展示

  • WB analysis using 30608-1-AP

    WB analysis using 30608-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30608-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 30608-1-AP

    FC experiment of Jurkat using 30608-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human BMPR1A (30608-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of U2OS using 30608-1-AP

    IF Staining of U2OS using 30608-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using BMPR1A antibody (30608-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30608-1-AP targets BMPR1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen BMPR1A fusion protein Ag32959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bone morphogenetic protein receptor, type IA
Synonyms ALK-3, ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3
Calculated Molecular Weight 532 aa, 60 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC028383
Gene Symbol BMPR1A
Gene ID (NCBI) 657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36894
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMPR1A (Bone morphogenetic protein receptor type-1A) is also named as ACVRLK3, ALK3 and SKR5. BMPR1A is an essential receptor for BMP signaling (PMID: 26279380). BMPR1A is necessary for chondrogenesis and osteogenesis (PMID: 32764110). BMPR1A is a cell surface marker of human SuSCs (PMID: 33658353).

实验方案

Product Specific Protocols
WB protocol for BMPR1A antibody 30608-1-APDownload protocol
IF protocol for BMPR1A antibody 30608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols