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验证数据展示

  • WB analysis using 30608-1-AP

    WB analysis using 30608-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30608-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 30608-1-AP

    FC experiment of Jurkat using 30608-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human BMPR1A (30608-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of U2OS using 30608-1-AP

    IF Staining of U2OS using 30608-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using BMPR1A antibody (30608-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

BMPR1A Polyclonal antibody

BMPR1A Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 30608-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

ALK-3, ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30608-1-AP targets BMPR1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 BMPR1A fusion protein Ag32959 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone morphogenetic protein receptor, type IA
别名 ALK-3, ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3
计算分子量 532 aa, 60 kDa
观测分子量 60-68 kDa
GenBank蛋白编号BC028383
基因名称 BMPR1A
Gene ID (NCBI) 657
RRIDAB_3086373
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36894
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMPR1A (Bone morphogenetic protein receptor type-1A) is also named as ACVRLK3, ALK3 and SKR5. BMPR1A is an essential receptor for BMP signaling (PMID: 26279380). BMPR1A is necessary for chondrogenesis and osteogenesis (PMID: 32764110). BMPR1A is a cell surface marker of human SuSCs (PMID: 33658353).

实验方案

Product Specific Protocols
WB protocol for BMPR1A antibody 30608-1-APDownload protocol
IF protocol for BMPR1A antibody 30608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols