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BMPR1A Polyclonal antibody

BMPR1A Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 30608-1-AP

Print datasheet

Synonyms

ALK-3, ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3

验证数据展示

  • WB analysis using 30608-1-AP

    WB analysis using 30608-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30608-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 30608-1-AP

    FC experiment of Jurkat using 30608-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human BMPR1A (30608-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of U2OS using 30608-1-AP

    IF Staining of U2OS using 30608-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using BMPR1A antibody (30608-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30608-1-AP targets BMPR1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen BMPR1A fusion protein Ag32959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bone morphogenetic protein receptor, type IA
Synonyms ALK-3, ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3
Calculated Molecular Weight 532 aa, 60 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC028383
Gene Symbol BMPR1A
Gene ID (NCBI) 657
RRIDAB_3086373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36894
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMPR1A (Bone morphogenetic protein receptor type-1A) is also named as ACVRLK3, ALK3 and SKR5. BMPR1A is an essential receptor for BMP signaling (PMID: 26279380). BMPR1A is necessary for chondrogenesis and osteogenesis (PMID: 32764110). BMPR1A is a cell surface marker of human SuSCs (PMID: 33658353).

实验方案

Product Specific Protocols
WB protocol for BMPR1A antibody 30608-1-APDownload protocol
IF protocol for BMPR1A antibody 30608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols