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验证数据展示

  • WB analysis using 82928-1-RR

    WB analysis using 82928-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82928-1-RR (BMPR1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 82928-1-RR

    FC experiment of MCF-7 using 82928-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BMPR1A Recombinant antibody (82928-1-RR, Clone:230137B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of HEK-293 using 82928-1-RR

    IP experiment of HEK-293 using 82928-1-RR

    IP result of anti-BMPR1A (IP:82928-1-RR, 4ug; Detection:82928-1-RR 1:2000) with HEK-293 cells lysate 1920 ug.

BMPR1A Recombinant antibody

BMPR1A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, ELISA
Cat No. 82928-1-RR

产品说明书

CloneNo. 230137B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), IP, ELISA

别名

ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3, Activin receptor like kinase 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, U2OS cells, HEK-293 cells, mouse heart tissue
Positive IP detected inHEK-293 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82928-1-RR targets BMPR1A in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 BMPR1A fusion protein Ag3410 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bone morphogenetic protein receptor, type IA
别名 ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3, Activin receptor like kinase 3
计算分子量 532 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC028383
基因名称 BMPR1A
Gene ID (NCBI) 657
RRIDAB_3670667
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36894
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMPR1A (Bone morphogenetic protein receptor type-1A) is also named as ACVRLK3, ALK3 and SKR5. BMPR1A is an essential receptor for BMP signaling (PMID: 26279380). BMPR1A is necessary for chondrogenesis and osteogenesis (PMID: 32764110). BMPR1A is a cell surface marker of human SuSCs (PMID: 33658353).

实验方案

Product Specific Protocols
WB protocol for BMPR1A antibody 82928-1-RRDownload protocol
IP protocol for BMPR1A antibody 82928-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols