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BMPR1A Recombinant antibody

BMPR1A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230137B7

Cat no : 82928-1-RR

Print datasheet

Synonyms

ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3, Activin receptor like kinase 3

验证数据展示

  • WB analysis using 82928-1-RR

    WB analysis using 82928-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82928-1-RR (BMPR1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 82928-1-RR

    FC experiment of MCF-7 using 82928-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BMPR1A Recombinant antibody (82928-1-RR, Clone:230137B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of HEK-293 using 82928-1-RR

    IP experiment of HEK-293 using 82928-1-RR

    IP result of anti-BMPR1A (IP:82928-1-RR, 4ug; Detection:82928-1-RR 1:2000) with HEK-293 cells lysate 1920 ug.

  • Affinity and Kinetic Characterization of 82928-1-RR

    Affinity and Kinetic Characterization of 82928-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82928-1-RR against Human BMPR1A were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inJurkat cells, U2OS cells, HEK-293 cells, mouse heart tissue
Positive IP detected inHEK-293 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82928-1-RR targets BMPR1A in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen BMPR1A fusion protein Ag3410 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name bone morphogenetic protein receptor, type IA
Synonyms ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3, Activin receptor like kinase 3
Calculated Molecular Weight 532 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC028383
Gene Symbol BMPR1A
Gene ID (NCBI) 657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36894
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMPR1A (Bone morphogenetic protein receptor type-1A) is also named as ACVRLK3, ALK3 and SKR5. BMPR1A is an essential receptor for BMP signaling (PMID: 26279380). BMPR1A is necessary for chondrogenesis and osteogenesis (PMID: 32764110). BMPR1A is a cell surface marker of human SuSCs (PMID: 33658353).

实验方案

Product Specific Protocols
WB protocol for BMPR1A antibody 82928-1-RRDownload protocol
IP protocol for BMPR1A antibody 82928-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols