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验证数据展示

  • WB analysis using 27413-1-AP

    WB analysis using 27413-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27413-1-AP (BMX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27413-1-AP

    IF Staining of HepG2 using 27413-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BMX antibody (27413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

BMX Polyclonal antibody

BMX Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27413-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

PSCTK3, PSCTK2, NTK38, ETK, EC:2.7.10.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, mouse epididymis tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27413-1-AP targets BMX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen BMX fusion protein Ag26407 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BMX non-receptor tyrosine kinase
Synonyms PSCTK3, PSCTK2, NTK38, ETK, EC:2.7.10.2
Calculated Molecular Weight 675 aa, 78 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC016652
Gene Symbol BMX
Gene ID (NCBI) 660
RRIDAB_3669599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMX (Bone marrow kinase on chromosome X) is a cytosolic tyrosine kinase and a member of the TEC kinase family. BMX is expressed in hematopoietic cells of the myeloid lineage where it participates in the immune response. Moreover, BMX is expressed in several types of cancers and very recently has been shown to mediate the survival and tumorigenicity of glioblastoma cancer stem cells (PMID: 22449076).

实验方案

Product Specific Protocols
WB protocol for BMX antibody 27413-1-APDownload protocol
IF protocol for BMX antibody 27413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols