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验证数据展示

  • WB analysis using 27413-1-AP

    WB analysis using 27413-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27413-1-AP (BMX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27413-1-AP

    IF Staining of HepG2 using 27413-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BMX antibody (27413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

BMX Polyclonal antibody

BMX Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27413-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

PSCTK3, PSCTK2, NTK38, ETK, EC:2.7.10.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, mouse epididymis tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27413-1-AP targets BMX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 BMX fusion protein Ag26407 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BMX non-receptor tyrosine kinase
别名 PSCTK3, PSCTK2, NTK38, ETK, EC:2.7.10.2
计算分子量 675 aa, 78 kDa
观测分子量70 kDa
GenBank蛋白编号BC016652
基因名称 BMX
Gene ID (NCBI) 660
RRIDAB_3669599
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMX (Bone marrow kinase on chromosome X) is a cytosolic tyrosine kinase and a member of the TEC kinase family. BMX is expressed in hematopoietic cells of the myeloid lineage where it participates in the immune response. Moreover, BMX is expressed in several types of cancers and very recently has been shown to mediate the survival and tumorigenicity of glioblastoma cancer stem cells (PMID: 22449076).

实验方案

Product Specific Protocols
WB protocol for BMX antibody 27413-1-APDownload protocol
IF protocol for BMX antibody 27413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols