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BN82002

BN82002 (CDC25 Phosphatase Inhibitor I) 是一种CDC25 phosphatase 家族的有效的、不可逆的、选择性的泛抑制剂。它显示出比 CD45 酪氨酸磷酸酶高约 20 倍的选择性。

CAS号

396073-89-5

分子式

C19H25N3O4

主要靶点

Phosphatase

仅限科研使用

Cat No : CM09886

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Synonyms

CDC25 Phosphatase Inhibitor I

验证数据展示

  • CAS No.: 396073-89-5

    BN82002
    CAS#: 396073-89-5

产品信息

BN82002 (CDC25 Phosphatase Inhibitor I) is a synthetic inhibitor of CDC25 phophatases

CAS号 396073-89-5
分子式 C19H25N3O4
主要靶点 Phosphatase
主要通路 代谢
分子量 359.42
纯度 99.91%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 CDC25 Phosphatase Inhibitor I

靶点活性

CDC25C-cat:4.6 μM|CDC25B3:6.3 μM|CDC25A:2.4 μM|Cdc25C:5.4 μM|Cdc25B2:3.9 μM

体外活性

The effect of BN82002 on cell proliferation is evaluated in vitro on several human tumor cell lines. Menadione is used as a control, that has been reported to inhibit cell proliferation. All of the examined cell lines are sensitive to BN82002 and Menadione in a concentration-dependent manner in the low micromolar range. The most sensitive is the pancreatic cancer cell line MIA PaCa-2 with an IC50 of 7.2 μM, and the less sensitive cell line is the colon cancer HT-29 with an IC50 of 32.6 μM. The range of activity is very similar to the one observed with menadione (5-15 μM). It is also showed that 50 μM BN82002 is a concentration that fully inhibits cell proliferation, the cell cycle distribution is only modestly affected with a slight decrease in S phase and an increase in cells containing both a G1 and a G2 DNA content, suggesting that the cells treated with BN82002 are arrested at various stages of the cell cycle.

溶解度

DMSO:150 mg/mL (417.34 mM)

参考文献

1.Brezak MC, et al. A novel synthetic inhibitor of CDC25 phosphatases: BN82002. Cancer Res. 2004 May 1;64(9):3320-5.

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