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BNIP3 Monoclonal antibody, PBS Only

BNIP3 Monoclonal Antibody for WB,IF,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB,IF,ELISA

Conjugate

Unconjugated

CloneNo.

2H7D8

Cat No : 68091-1-PBS

Print datasheet

Synonyms

BNIP3, NIP3

验证数据展示

  • WB analysis using 68091-1-Ig (same clone as 68091-1-PBS)

    WB analysis using 68091-1-Ig (same clone as 68091-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68091-1-Ig (same clone as 68091-1-PBS)

    IF Staining of HepG2 using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using BNIP3 antibody (68091-1-Ig, Clone: 2H7D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

产品信息

68091-1-PBS targets BNIP3 in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB,IF,ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen BNIP3 fusion protein Ag27935 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name BCL2/adenovirus E1B 19kDa interacting protein 3
Synonyms BNIP3, NIP3
Calculated Molecular Weight 194 aa, 22 kDa
Observed Molecular Weight25-30,50-60 kDa
GenBank Accession NumberBC009342
Gene Symbol BNIP3
Gene ID (NCBI) 664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12983
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.