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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 68118-1-Ig (same clone as 68118-1-PBS)

    WB analysis using 68118-1-Ig (same clone as 68118-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68118-1-Ig (BNIP3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 68118-1-Ig (same clone as 68118-1-PBS)

    IHC staining of human prostate cancer using 68118-1-Ig (same clone as 68118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BNIP3L antibody included
  • IHC staining of rat kidney using 68118-1-Ig (same clone as 68118-1-PBS)

    IHC staining of rat kidney using 68118-1-Ig (same clone as 68118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BNIP3L antibody included
  • IHC staining of mouse brain using 68118-1-Ig (same clone as 68118-1-PBS)

    IHC staining of mouse brain using 68118-1-Ig (same clone as 68118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BNIP3L antibody included
  • IF Staining of HeLa using 68118-1-Ig (same clone as 68118-1-PBS)

    IF Staining of HeLa using 68118-1-Ig (same clone as 68118-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BNIP3L antibody (68118-1-Ig, Clone: 1F6A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

BNIP3L Monoclonal antibody, PBS Only

BNIP3L Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68118-1-PBS

产品说明书

CloneNo. 1F6A3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

BNIP3H, BNIP3A, BNIP, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, Adenovirus E1B19K-binding protein B5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BNIP3L Ready-To-Use IHC Kit. BNIP3L primary antibody included.

产品信息

68118-1-PBS targets BNIP3L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BNIP3L fusion protein Ag31547 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 BCL2/adenovirus E1B 19kDa interacting protein 3-like
别名 BNIP3H, BNIP3A, BNIP, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, Adenovirus E1B19K-binding protein B5
计算分子量 24 kDa
观测分子量35 kD
GenBank蛋白编号BC001559
基因名称 BNIP3L
Gene ID (NCBI) 665
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60238
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BNIP3L/Nix is a mitochondrial protein from the outer membrane that belongs to the BH3-only protein from the BCL2 family. BNIP3L was initially recognized as a proapoptotic protein with milder efficacy in inducing apoptosis compared to other proteins in this family. These phenotypes raised concerns about the molecular function of BNIP3L besides inducing apoptosis. Moreover, studies have shown that BNIP3L is a 24-kDa protein, which is predominantly expressed as a 48-kDa dimer. When analyzed by SDS-PAGE, BNIP3 migrates predominantly as a about 60 kDa dimer in addition to the 30 kDa monomer. (PMID: 34930907, PMID: 32286918)